Strain-specific variations in Toxoplasma gondii GRA1, GRA5, GRA6, GRA8, and GRA14
نویسندگان
چکیده
منابع مشابه
Selective and strain-specific NFAT4 activation by the Toxoplasma gondii polymorphic dense granule protein GRA6
Toxoplasma gondii infection results in co-option and subversion of host cellular signaling pathways. This process involves discharge of T. gondii effector molecules from parasite secretory organelles such as rhoptries and dense granules. We report that the T. gondii polymorphic dense granule protein GRA6 regulates activation of the host transcription factor nuclear factor of activated T cells 4...
متن کاملCloning, expression and immunoreactivity of recombinant Toxoplasma gondii GRA5 protein
BACKGROUND AND OBJECTIVES Toxoplasma gondii is an obligatory intracellular parasite which causes severe diseases in the fetus of pregnant women and immunocopmromised patients. Serological tests based on recombinant protein are one of the main diagnosis methods for the detection of specific antibodies in serum samples. Dense granule antigenic proteins derived from T. gondii (TgGRAs) are potentia...
متن کاملBacterial production of dense granule antigen GRA8 of Toxoplasma gondii.
BACKGROUND Dense granule antigens (GRA antigens) of Toxoplasma gondii induce strong antibody response in humans and are considered as useful diagnostic antigens. Previous studies reported expression of amino terminal GRA8 protein in fusion with large tags such as glutathione-S-transferase. The present study aimed to produce soluble full length immunogenic GRA8 in bacteria. METHODS GRA8 comple...
متن کاملClone of the gene of the dense granule antigen (GRA6) of the Toxoplasma gondii pig strain.
The gene of GRA6 of Toxoplasma gondii pig strain was cloned; and the value of recombinant GRA6 as a diagnostic antigen of toxoplasmosis in China was investigated. A pair of primers was synthesized according to the DNA sequence of GRA6 of T. gondii RH strain, and the DNA of pig strain tachyzoites was prepared. A single, specific DNA fragment was successfully amplified from the genomic DNA of tac...
متن کاملIdentifying Novel B Cell Epitopes within Toxoplasma gondii GRA6
The study of antigenic epitopes from Toxoplasma gondii has not only enhanced our understanding of the structure and function of antigens, the reactions between antigens and antibodies, and many other aspects of immunology, but it also plays a significant role in the development of new diagnostic reagents and vaccines. In the present study, T. gondii GRA6 epitopes were identified using bioinform...
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ژورنال
عنوان ژورنال: IOP Conference Series: Materials Science and Engineering
سال: 2016
ISSN: 1757-8981,1757-899X
DOI: 10.1088/1757-899x/107/1/012055